Anti-HA−TRITC antibody, Mouse monoclonal
SIGMA/H9037 - ~1 mg/mL, clone HA-7, purified from hybridoma cell culture
Synonym: Monoclonal Anti-HA; Anti-HA; Anti-Influenza Hemagglutinin
MDL Number: MFCD00803873
Product Type: Chemical
Immunofluorescence 293T cells expressing HA fusion protein were fixed and permeabilized with 3% to 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 15 μg/mL Monoclonal Anti-HA-TRITC antibody produced in Mouse, Clone: HA-7 (Cat. No. H9037).
| antibody form | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HA-7, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | TRITC conjugate |
| form | buffered aqueous solution |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage condition | protect from light |
| storage temp. | −20°C |
| technique(s) | direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Application: | Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice. |
| Biochem/physiol Actions: | The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells. |
| General description: | Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins. |
| Immunogen: | synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH |
| Physical form: | Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |