Vascular Endothelial Growth Factor 165 human
SIGMA/H9166 - VEGF165, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture
Synonym: Endothelial Growth Factor; Vascular Growth Factor
MDL Number: MFCD00286542
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| assay | ≥95% (SDS-PAGE) |
| biological source | human |
| form | lyophilized powder |
| impurities | ≤1 EU/μg |
| manufacturer/tradename | HumanZyme |
| mol wt | dimer 45 kDa (glycosylated) |
| packaging | pkg of 10 μg |
| pkg of 5x10 μg | |
| potency | ≤5 ng/mL EC50 |
| quality | endotoxin tested |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P15692  |
| Analysis Note: | The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells). |
| Biochem/physiol Actions: | VEGF (vascular endothelial growth factor) acts as a potent angiogenic factor and mitogen that stimulates proliferation, migration and formation of endothelial cells. VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system. VEGFA is known to interact with VEGFR co-receptors such as heparan sulphate proteoglycans and neuropilins. Mutation in the VEGFA gene results in defective vascular development. VEGF is induced by hypoxia, oncogene mutations, and cytokines such as (interleukin) IL-1, IL-8, TNF-α (tumor necrosis factor α). |
| General description: | Vascular endothelial growth factor 121 (VEGF121) belongs to the PDGF(platelet derived growth factor)/VEGF growth factor family. The VEGFA gene is mapped to human chromosome 6p21.1. The encoded protein is a dimeric glycoprotein consisting two monomers antiparallel to each other. There are five types of VEGF ligands identified in mammals, which exists as different splice variants. Vascular endothelial growth factor 165 (VEGF165) belongs to the PDGF(platelet derived growth factor)/VEGF growth factor family and VEGF is secreted by many cell types.HumanKine VEGF165 is expressed in human HEK 293 cells as a glycosylated homodimer with an apparent molecular mass of 45 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. |
| Legal Information: | HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc |
| Packaging: | 10 μg in poly bottle |
| Physical form: | Lyophilized from a 0.2 μm filtered solution of 1X PBS |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |