Histone from calf thymus
SIGMA/H9250 - Type II-A, lyophilized powder
Synonym: histone type VIII-S
CAS Number: 9064-47-5
MDL Number: MFCD00131329
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H9250-100MG
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: H9250-500MG
| application(s) | cell analysis |
| biological source | bovine (calf) thymus |
| form | lyophilized powder |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| type | Type II-A |
| Application: | Histone from calf thymus has been used: • in methyltransferase reaction mixture for methyltransferase assay • in in vitro binding assay, to explore the potential binding of the MORF4 (mortality factor on chromosome 4) - related gene on chromosome 15 (MRG15) chromo domain with histone • in in vitro coculture assays, to stimulate liver resident Kupffer cells |
| Biochem/physiol Actions: | The core histones are involved in histone-histone interactions and maintains the organization of the nucleosomal DNA. The histone tail helps in forming higher-order nucleosomal structures. The overall structure and action of chromatin fiber is regulated by histone modifications. |
| Features and Benefits: | Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers. |
| General description: | Histones are octameric proteins containing H3, H4, H2A and H2B. It is the core protein around which DNA is wound forming the nucleosome structure. The core histone proteins contain globular domains. Each protein is composed of basic amino acids at its N-terminal. The H2A has an extra amino acid at its C-terminal end forming a histone tail. The nucleosome structure is stabilized by the linker histones during chromatin condensation. |
| Packaging: | 100, 500 mg in poly bottle |
| Preparation Note: | Unfractionated whole histone |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106305 |