Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit
SIGMA/H9286 - 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-H3K9ac
MDL Number: MFCD02685921
Product Type: Chemical
Immunohistochemistry Anti-Acetyl-Histone H3 [Ac-Lys9]: Cat. No. H9286: Immunoperoxidase staining of Anti-Acetyl-Histone H3 [Ac-Lys9] in formalin fixed, paraffin-embedded rat liver at 0.1 μg/mL.
Immunohistochemistry Anti-Acetyl-Histone H3 [Ac-Lys9]: Cat. No. H9286: Immunoperoxidase staining of Anti-Acetyl-Histone H3 [Ac-Lys9] in formalin fixed, paraffin-embedded rat lung at 0.1 μg/mL.
Immunohistochemistry Anti-Acetyl-Histone H3 [Ac-Lys9]: Cat. No. H9286: Immunoperoxidase staining of Anti-Acetyl-Histone H3 [Ac-Lys9] in formalin fixed, paraffin-embedded mouse testis at 0.1 μg/mL.
Immunohistochemistry Anti-Acetyl-Histone H3 [Ac-Lys9]: Cat. No. H9286: Immunoperoxidase staining of Anti-Acetyl-Histone H3 [Ac-Lys9] in formalin fixed, paraffin-embedded mouse liver at 0.1 μg/mL.
Immunoblot
Whole cell extract from serum and sodium butyrate-treated NIH3T3 cells was separated on SDS-PAGE and blotted with Anti-Acetyl-Histone H3 [Ac-Lys9] (Cat. No. H9286). The antibodies were developed with Goat Anti-Rabbit IgG-Alkaline phosphatase (Cat. No. A9919) and SIGMA FAST™ BCIP/NBT substrate (Cat. No. B5655). Inhibition with the acetylated [Ac-Lys9] histone H3 peptide (Lane 2) with no inhibition with the non-acetyl-histone H3 peptide (Lane 3).
Lane 1: Anti-Acetyl-Histone H3 [Ac-Lys9]
Lane 2: Anti-Acetyl-Histone H3 [Ac-Lys9] + [Ac-Lys9] histone H3 peptide
Lane 3: Anti-Acetyl-Histone H3 [Ac-Lys9] + [non-Ac] histone H3 peptide
Whole cell extract from serum and sodium butyrate-treated NIH3T3 cells was separated on SDS-PAGE and blotted with Anti-Acetyl-Histone H3 [Ac-Lys9] (Cat. No. H9286). The antibodies were developed with Goat Anti-Rabbit IgG-Alkaline phosphatase (Cat. No. A9919) and SIGMA FAST™ BCIP/NBT substrate (Cat. No. B5655). Inhibition with the acetylated [Ac-Lys9] histone H3 peptide (Lane 2) with no inhibition with the non-acetyl-histone H3 peptide (Lane 3).
Lane 1: Anti-Acetyl-Histone H3 [Ac-Lys9]
Lane 2: Anti-Acetyl-Histone H3 [Ac-Lys9] + [Ac-Lys9] histone H3 peptide
Lane 3: Anti-Acetyl-Histone H3 [Ac-Lys9] + [non-Ac] histone H3 peptide
Immunofluorescence NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:1,000 Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in Rabbit (Cat. No. H9286) followed by 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887). Picture C: cells were counterstained with DAPI (blue) to stain nuclei (Picture A: without DAPI, Picture B: only DAPI).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2-2.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~17 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken |
| storage temp. | −20°C |
| target post-translational modification | acetylation (Lys9) |
| technique(s) | indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate |
| microarray: suitable | |
| western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate | |
| UniProt accession no. | P68431  |
| Application: | Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in: • indirect immunofluorescence • immunoblotting • immunohistochemistry |
| Biochem/physiol Actions: | Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers. |
| Biochem/physiol Actions: | In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for: • indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate • microarray • western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate |
| Immunogen: | synthetic acetylated [Ac-Lys9] histone H3 peptide (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species including mouse, rat, bovine, chicken, frog, Drosophila, and C. elegans, and is highly conserved (single amino acid substitution) in Tetrahymena histone H3. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |