Hanks’ Balanced Salt solution
SIGMA/H9394 - Modified, with sodium bicarbonate, without calcium chloride and magnesium sulfate, liquid, sterile-filtered, suitable for cell culture
Synonym: HBSS
Product Type: Chemical
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| components | NaHCO3: 0.35 g/L glucose: 1.0 g/L (Dextro) phenol red: 0.011 g/L |
| form | liquid |
| impurities | endotoxin, tested |
| Quality Level | 400  |
| shipped in | ambient |
| sterility | sterile-filtered |
| technique(s) | cell culture | mammalian: suitable |
| Application: | Hanks′ Balanced Salt solution has been used: • as a component of the culture medium for culturing of mouse cortical slices • as a component of the dissociation buffer for incubation of human-induced pluripotent stem cells (hiPSCs) and hiPSC-derived cardiomyocytes (CMs) • for in situ perfusions of mice liver |
| Biochem/physiol Actions: | Balanced salt solution in cell culture plays multiple roles such as, an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance, and provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It is also involved in providing a buffering system to maintain the medium within the physiological pH range (7.2-7.6) and provides the principal energy source for cell metabolism. |
| General description: | Hanks′ Balanced Salt solution is recommended for suspension cultures or whenever cell clumping is a problem. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 12352207 |