β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei
SIGMA/H9408 - lyophilized powder, ≥200 units/mg protein
Synonym: (R)
CAS Number: 9028-38-0
MDL Number: MFCD00130633
Product Type: Chemical
Unit Definition One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.
| biological source | bacterial (Pseudomonas lemoignei) |
| foreign activity | lactic dehydrogenase ≤0.05% |
| malic dehydrogenase ≤0.1% | |
| form | lyophilized powder |
| purified by | chromatography |
| Quality Level | 200  |
| specific activity | ≥200 units/mg protein |
| storage temp. | −20°C |
| Application: | Suitable for the determination of acetoacetate and |
| Application: | β-Hydroxybutyrate Dehydrogenase from Pseudomonas lemoignei has been used in coupled assay to confirm the formation of acetoacetate. |
| Biochem/physiol Actions: | In Paracoccus denitrificans, 3-hydroxybutyrate dehydrogenase plays a key role in the degradation of intracellular polyhydroxybutyrate and polyhydroxyvalerate. |
| Biochem/physiol Actions: | In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle). |
| General description: | D-β-hydroxybutyrate dehydrogenase (BDH) is a membrane bound lipid-requiring enzyme. |
| Other Notes: | One unit will oxidize 1.0 μmole of |
| Physical form: | lyophilized powder containing sucrose, β-NAD and Tris buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥200 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.1.30 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |