Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit
SIGMA/H9411 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-AHO3; Anti-BDMR; Anti-HA6116; Anti-HD4; Anti-HDAC-4; Anti-HDAC-A; Anti-HDACA
MDL Number: MFCD03791601
Product Type: Chemical
Enhanced Validation-By Independent Antibodies HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton. Fixed cells were stained with 1:250 Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in Rabbit (Cat. No. H9411) (A) and developed using Goat Anti-Rabbit IgG, Cy3 conjugate, and 1:250 Anti-Histone Deacetylase 4 (HDAC4) antibody, Mouse monoclonal, Clone: HADC4-144 (Cat. No. H0163) (B). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate antibody. Results Two Anti-Histone Deacetylase 4 (HDAC4), H9411 (A) And H0163 (B) target different regions of Histone Deacetylase 4 (HDAC4) show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1:250 Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in Rabbit (Cat. No. H9411). The antibody was developed using Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat (Cat. No. F9887).
Western Blotting Whole extract of 3T3 cells were separated on SDS-PAGE and probed with Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in Rabbit (Cat. No. H9411). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:1,000 antibody 2. 1:2,000 antibody 3. Negative Control
Western Blotting Whole extract of Rat brain extract were separated on SDS-PAGE and probed with Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in Rabbit (Cat. No. H9411). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. Negative Control
Immunoprecipitation Rabbit Anti-HDAC4 (ML-19) (Cat. No. H9411) was used to immunoprecipitate HDAC4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-HDAC4 (ML-19) (Cat. No. H9411). Lanes 1. 10 μg antibody 2. 5 μg antibody 3. Negative control: No cell extract 4. Positive control: HeLa whole cell extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~140 kDa (additional weaker bands may be present) |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using RIPA extract of HeLa nuclei |
| indirect immunofluorescence: 1:250 using HeLa cells | |
| microarray: suitable | |
| western blot: 1:1,000 using whole extracts of mouse NIH3T3 cells | |
| western blot: 1:500 using whole extracts of rat brain | |
| UniProt accession no. | P56524  |
| Application: | Anti-Histone Deacetylase 4 (HDAC4) (ML-19) antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunofluorescence • immunostaining • immunohistochemistry • chromatin immunoprecipitation |
| Biochem/physiol Actions: | Histone deacetylase 4 (HDAC4) exhibits intrinsic nuclear import and export signals for its dynamic nucleocytoplasmic shuttling. Its association with 14-3-3 and monocyte enhancer factor 2 (MEF2) proteins can disturb shuttling and thus directs HDAC4 to the cytoplasm and the nucleus, respectively. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Mammalian histone deacetylases (HDACs) can be divided into three classes according to sequence homology. Class II consists of the yeast Hda1-like proteins histone deacetylase 4,5,6,7,8,9, 10 (HDAC4, HDAC5, HDAC6, HDAC7, HDAC9, and HDAC10. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 1-19 of human HDAC4 with C-terminal added lysine conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |