Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in rabbit
SIGMA/H9536 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-HDAC4
MDL Number: MFCD03791600
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Immunofluorescence. Antibody recognition: Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in Rabbit (Cat. No. H9536) is confirmed by immunofluorescence in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing HADC-4 (Picture A, B) while no specific staining is detected in the wild type sample-HEK-293T (Picture C, D). Pictures B and D: cells were counterstained with DAPI (blue) to stain nuclei. Antibody dilution: 1:250.
Western Blotting Rat brain extract were separated on SDS-PAGE and probed with Anti-Histone Deacetylase 4 (HDAC4) (DM-15) antibody produced in Rabbit (Cat. No. H9536). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:500 antibody 2. 1:1,000 antibody 3. Negative Control
Immunoprecipitation Rabbit Anti-HDAC4 (DM-15) (Cat. No. H9536) was used to immunoprecipitate HDAC4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-HDAC4 (DM-15) (Cat. No. H9536). Lanes 1. 10 μg antibody 2. 5 μg antibody 3. Negative control: No cell extract 4. Positive control: HeLa whole cell extract
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~140 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10-20 μg using RIPA extract of HeLa nuclei |
| indirect immunofluorescence: 1:250 using HEK 293T cells expressing recombinant mouse HDAC4 | |
| microarray: suitable | |
| western blot: 1:1,000 using whole extracts of mouse NIH3T3 cells | |
| western blot: 1:500 using whole extracts of rat brain | |
| UniProt accession no. | P56524  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below: • Western Blotting • Immnofluorescence • Immunoprecipitation |
| Biochem/physiol Actions: | Anti-Histone Deacetylase 4 (HDAC4), recognizes human, mouse and rat HDAC4. |
| Biochem/physiol Actions: | Histone deacetylase 4 (HDAC4) contains import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor myocyte enhancer factor-2 (Mef2) protein and 14-3-3. This interaction disturbs nucleocytoplasmic shuttling and then guides HDAC4 to the cytoplasm and the nucleus, respectively. In addition, HDAC4 with HDAC5 and HDAC7 represses transcription by binding to the nuclear receptor co-repressors silencing mediator of retinoic acid and thyroid hormone receptors (SMRT) and nuclear receptor co-repressor (NCOR). |
| Biochem/physiol Actions: | Studies show that HDAC4 contains a composed import and export nuclear signal that aids in nucleocytoplasmic shuttling. It has physical and functional interactions with transcription factor MEF2C and 14-3-3. This interaction results in the shuttling and it guides HDAC4 to the cytoplasm and the nucleus.The C-terminal domain of HDAC4 shows innate histone deacetylase activity. It is seen that MEF2C combines with HDAC4 at the N-terminal domain and downmodulate c-jun promoter activity. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Histone deacetylases (HDACs) are competing enzymes, belonging to histone deacetylase family. There are two classes of HDACs with six to seven different types of HDACs proteins. HDAC1,HDAC2 and HDAC3 belong to Class I HDACs and HDAC4, HDAC6, and HDAC7 belong to Class II HDACs. Class I HDACs consists of a single deacetylase domain at the N-termini and diversified C-terminal regions, while Class II contains a deacetylase domain at C-terminal position. |
| General description: | Mammalian histone deacetylases (HDACs) are classified into three classes according to sequence homology. HDAC1, HDAC2, HDAC3 and HDAC8 belong to class I HDACs and HDAC4, HDAC6, and HDAC7 belong to class II HDACs. Class III consists of the yeast sirtuins (sir2-like protein). Class I HDACs are ubiquitously expressed. Whereas, most of the class II HDACs show tissue-specific expression. The antibody is affinity-purified using the immunizing peptide immobilized on agarose. |
| Immunogen: | Synthetic peptide corresponding to amino acid residues of human HDAC4 with C-terminal added lysine conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8°C for up to one month. For prolonged storage, freeze in working aliquots at -20°C. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |