Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit
SIGMA/H9539 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Amphoterin; Anti-High mobility group box 1; Anti-High mobility group protein 1
MDL Number: MFCD02263062
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunofluoresence. PC12 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1 μg/mL Anti-HMGB1 (HMG1) (C-terminal) antibody produced in Rabbit (Cat. No. H9539) (A). The antibody developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody, and 1 μg/mL Anti-HMGB1 (HMG1) (N-terminal) antibody produced in Rabbit (Cat. No. H9664) (B). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 Conjugate antibody. Results Two Anti HMGB1 (HMG1) antibodies, H9539 (A) and H9664 (B) target different regions of HMGB1 (HMG1) show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunoblotting RIPA cell lysates of various tissues and cell lines were separated SDS-PAGE, blotted with 2 mg/mL Rabbit Anti-HMGB1 (C-terminal) (Cat. No. H9539) and developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) using a chemiluminescence substrate. Lanes 1. Mouse kidney 2. NIH3T3 3. Rat1 4. RINSH 5. PC12 6. K562 7. HEK293T 8. HeLa nuclear 9. HeLa cell lysate
Immunoprecipitation Lane 1. 5 μg of Rabbit Anti-HMGB1 (HMG1) (C-Terminal) (Cat. No. H9539) was used to immunoprecipitate human HMGB1 from HEK-293T cell lysate. Detection antibody: Rabbit Anti-HMGB1 (HMG1) (N-Terminal) (Cat. No. H9664). Lane 2. Negative control – without cell lysate. Lane 3. Negative control – without IP antibody.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 25 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates. |
| indirect immunofluorescence: suitable | |
| western blot: 0.5-1 μg/mL using 3T3 or PC12 cell lysates. | |
| UniProt accession no. | P09429  |
| Application: | Anti-HMGB1 (HMG1) (C-terminal) antibody produced in rabbit has been used in ELISA and immunofluorescence. |
| Biochem/physiol Actions: | In the nucleus, HMGB1 (high-mobility group protein B1) works as a nucleosome stabilizer and transcriptional regulator. It also exhibits chaperone-like activity and suppresses aggregation of polyglutamine. It has a role in inflammation and autophagy. HMGB1 can interact with toll-like receptor (TLR) 2 and 4, interleukin-1β, chemokine (C-X-C motif) ligand 12 (CXCL12) and pathogen-associated molecular pattern (PAMP) molecules. It also works as a damage-associated molecular pattern molecule (DAMP). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The High Mobility Group (HMG1) is secreted by activated monocytes and macrophages. The gene is mapped to human chromosome 13q12. |
| Immunogen: | synthetic peptide corresponding to amino acid 165-180 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat. |
| Other Notes: | In order to obtain the best results using various techniques and preparations, we recommend determining the optimal working dilutions by titration. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |