Monoclonal Anti-HA antibody produced in mouse
SIGMA/H9658 - clone HA-7, ascites fluid
Synonym: Monoclonal Anti-HA; HA Antibody - Monoclonal Anti-HA antibody produced in mouse; Ha Antibody Sigma; Sigma Ha Antibody; Anti-HA; Anti-Influenza Hemagglutinin
MDL Number: MFCD00803873
Product Type: Chemical
Western Blotting JOURNAL CITATION: TORC1 signaling inhibition by rapamycin and caffeine affect lifespan, global gene expression, and cell proliferation of fission yeast. By: Rallis, C., Codlin, S., et al. in Aging Cell, 2013. PubMed ID: 23551936 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1111/acel.12080), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: TORC1 signaling inhibition by rapamycin and caffeine affect lifespan, global gene expression, and cell proliferation of fission yeast. By: Rallis, C., Codlin, S., et al. in Aging Cell, 2013. PubMed ID: 23551936 Image collected and cropped by CiteAb from the following publication, (http://doi.wiley.com/10.1111/acel.12080), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: Dual effects of TGF-beta on ERalpha-mediated estrogenic transcriptional activity in breast cancer. By: Ren, Y., Wu, L., et al. in Mol Cancer, 2009. PubMed ID: 19943940 Image collected and cropped by CiteAb from the following publication, (http://molecular-cancer.biomedcentral.com/articles/10.1186/1476-4598-8-111), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Monoclonal Anti-HA: Cat. No. H9658: Staining of HEK293T cells transfected with a recombinant protein HA tagged, detected with Anti-HA Mouse primary antibody and Anti-Mouse Alexa543 secondary antibody. Microtubules detected with Anti-α-tubulin Mouse primary antibody and Anti-Mouse Alexa488 secondary antibody. Nucleus seen thanks to DAPI staining. Confocal image. Submitted by Dr L. Francelle, Commissariat d′Energie Atomique.
Immunoblotting Whole extract of human HEK-293T cells overexpressing N-terminal HA tagged fusion protein was separated on SDS-PAGE and probed with Monoclonal Anti-HA Clone: HA7 (Cat. No. H9658). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:10,000 2. Antibody dilution 1:20,000 3. Antibody dilution 1:40,000
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | HA-7, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect ELISA: suitable | |
| western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein |
| Application: | Monoclonal Anti-HA antibody produced in mouse has been used in 293T cells for: • immunoprecipitation in human embryonic kidney 293 cells • immunoblotting in HeLa cells • immunopurification in human embryonic kidney 293 cells • immunostaining for electron microscopy in mouse embryonic fibroblasts • in immunocytochemistry of hippocampal slices |
| Application: | Monoclonal Anti-HA antibody produced in mouse has been used in: • Immunoblotting, to study the expression of fusion protein • Coimmunoprecipitation assay • Immunoblotting of lysate protein |
| Biochem/physiol Actions: | Hemagglutinin (HA) is the surface glycoprotein of influenza virus. HA is crucial for virus entry and interaction with N-acetylneuraminic acid (Neu5Ac) in host cell surface. HA coordinates with neuraminidase (NA) and facilitates viral entry into host. HA exists as a trimer and each monomer has stalk transmembrane and globular regions. The globular region contains carbohydrate side chains crucial for infecting host. |
| Biochem/physiol Actions: | Influenza virus hemagglutinin (HA) acts as a key immunogen in avian influenza virus (AIV) vaccines. Activated HA facilitates virus attachment to oligosaccharide receptors on host cell surface and is involved in penetration through fusion following conformational modifications in the endolysosomal compartment. 226 and 228 residues in hemagglutinin (HA) of the H3 subtype play a key role in host range restriction and receptor specificity. Influenza virus hemagglutinin (HA) is responsible for 1918 influenza pandemic. |
| Biochem/physiol Actions: | The antibody recognizes native as well as denatured-reduced forms of HA-tagged proteins and is reactive with N- or C-terminal HA-tagged fusion proteins expressed in E. coli or in mammalian cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The gene encoding influenza virus hemagglutinin (HA) precursor is mapped on segment 4. It encodes a 76kDa surface glycoprotein which exists as trimers on the viral envelope. |
| Immunogen: | synthetic peptide corresponding to a fragment of human influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Solutions at working dilution should be discarded if not used within 12 hours. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |