Anti-HMGB2 (HMG2) antibody produced in rabbit
SIGMA/H9789 - affinity isolated antibody
Synonym: Anti-High mobility group box 2; Anti-High mobility group protein 2
Product Type: Chemical
Immunofluorescence Nuclear localization of HMGB2 in PC12 cells. A. Cells were stained with Rabbit Anti-HMGB2 (Cat. No. H9789) at 1 μg/mL B. DAPI staining C. Merged staining of HMGB2 and DAPI indicating nuclear staining of HMGB2
Immunofluorescence Anti-HMGB2: Cat. No. E9789: Immunofluorescence of HUVEC cells using Anti-HMGB2 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Immunofluorescence Graph Anti-HMGB2: Cat. No. E9789: Intensity analysis of Anti-HMGB2 staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
Immunoprecipitation Lanes 1: 5 μg of Rabbit Anti-HMGB2 (Cat. No. H9789) were used to immunoprecipitate HMGB2 from HEK-293T cell lysate 2: Negative control – without cell lysate 3: Negative control – without antibody 4: Positive control – Extract of HEK-293Tdirect labeling Detection antibody: Rabbit Anti-HMGB2 (Cat. No. H9789)
Immunoblotting Lysates of various cell lines were separated SDA-PAGE, blotted with 2 mg/mL Rabbit Anti-HMGB2 (Cat. No. H9789) and developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) using a chemiluminescence substrate. Lanes 1. NIH3T3 2. Rat1 3. RINSH 4. PC12 5. K562 6. HEK-293T 7. HeLa nuclear 8. HeLa
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates. |
| indirect immunofluorescence: 1-2 μg/mL using fixed PC12 cultured cells. | |
| western blot: 2-4 μg/mL using PC12 whole cell lysates. | |
| UniProt accession no. | P26583  |
| Application: | Anti-HMGB2 (HMG2) antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunofluorescence |
| Application: | Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol . |
| Biochem/physiol Actions: | The high-mobility group box 1 and 2 (HMGB 1 & 2) proteins participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signalling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The high mobility group (HMG) proteins are subdivided into 3 families based on their functional sequence motif characteristic: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) and the HMGA (formerly HMG-I/Y/C) family. The HMGB protein family, including HMGB1, HMGB2, and HMGB3, are highly conserved (more than 80% amino acid identity), and indistinguishable in their biochemical properties. The high-mobility group box 2 (HMGB2) is a ~25 kDa protein of 209 amino acids that consists of two homologous HMG-boxes rich in basic amino acids and an acidic tail at the carboxy-terminus. HMGB2 is restricted mainly to lymphoid organs and testes, although it is widely expressed in embryogenesis. |
| Immunogen: | synthetic peptide corresponding to amino acid 165-181 of human HMGB2, conjugated to KLH. The corresponding sequence is conserved in mouse and differs by one amino acid in rat. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |