HIS-Select® Nickel Magnetic Agarose Beads
SIGMA/H9914
Synonym: nickel charged magnetic beaded agarose
Product Type: Chemical
| capacity | ≥15 mg/mL binding capacity |
| conjugate | magnetic beads |
| form | suspension |
| matrix | 6% beaded magnetic agarose |
| Quality Level | 200  |
| shelf life | 2 yr (Unopened product) |
| storage temp. | 2-8°C |
| Application: | The HIS-Select Nickel Magnetic Agarose Beads are designed for use in automated and small-scale affinity capture (molecular pull-down) purifications of histidine-tagged protein / His-tag protein while exhibiting low non-specific binding of other proteins. The beads can be used to purify His-tagged proteins under native and denaturing conditions. |
| General description: | HIS-Select Magnetic Agarose Beads consist of paramagnetic, immobilized metal-ion affinity chromatography (IMAC) resin that contain a proprietary quadridentate chelate, which is bound with nickel and covalently attached through a non-charged, hydrophilic linker to magnetic beaded agarose. The magnetic properties of the beads aid in manipulations, such as repetitive washings, and recovery of the protein bound beads. This leads to greater experimental reproducibility and more accurate quantitation of the His-tagged proteins of interest. |
| Legal Information: | HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Physical form: | Supplied as a 50% slurry suspension in 30% Ethanol. |
| Symbol |    GHS02,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H317 - H350 - H360D - H373 - H412 |
| Precautionary statements | P201 - P210 - P273 - P280 - P303 + P361 + P353 - P308 + P313 |
| Hazard Codes | T |
| Risk Statements | 49-61-10-43-48/20-52/53 |
| Safety Statements | 53-36/37-45-61 |
| RIDADR | UN1170 - class 3 - PG 2 - Ethanol, solution |
| WGK Germany | WGK 3 |
| Flash Point(F) | 86.0 °F |
| Flash Point(C) | 30 °C |
| Storage Temp. | 2-8°C |
| UNSPSC | 41106500 |