Anti-GPKOW antibody produced in rabbit
SIGMA/HPA000287 - Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-G patch domain and KOW motifs-containing protein antibody produced in rabbit; Anti-G patch domain-containing protein 5 antibody produced in rabbit; Anti-Protein MOS2 homolog antibody produced in rabbit; Anti-Protein T54 antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, colon, kidney and pancreas using Anti-GPKOW antibody HPA000287 (A) shows similar protein distribution across tissues to independent antibody HPA001894 (B).
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-GPKOW antibody HPA000287 (A) shows similar pattern to independent antibody HPA001894 (B).
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows moderate to strong nuclear positivity in neurons and i glial cells.
Immunohistochemistry Immunohistochemical staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate to strong nuclear positivity in cells in tubules and in cells in glomeruli.
Immunohistochemistry Immunohistochemical staining of human pancreas shows moderate to strong nuclear positivity in exocrine glandular cells and in islets of Langerhans.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex using Anti-GPKOW antibody HPA000287.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-GPKOW antibody HPA000287.
Immunohistochemistry Immunohistochemical staining of human kidney using Anti-GPKOW antibody HPA000287.
Immunohistochemistry Immunohistochemical staining of human pancreas using Anti-GPKOW antibody HPA000287.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Western Blotting Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | ELQSVKPQEAPKELVIPLIQNGHRR |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:20-1:50 | |
| UniProt accession no. | Q92917  |
| Application: | Anti-GPKOW antibody has been used in Western blotting. All Prestige Antibodies®Powered by Atlas Antibodies is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | G-patch (glycine-rich) domain and KOW (Kyrpides, Ouzounis and Woese) domain (GPKOW) plays a crucial role in both alternative splicing and miRNA processing. This RNA binding protein acts as a cofactor for DHX16 (DEAH (Asp-Glu-Ala-His) box polypeptide 16 protein) activity in the spliceosome. The encoded protein also helps in suppressing the splicing defect caused by mutation of DHX16 gene. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | G-patch (glycine-rich) domain and KOW (Kyrpides, Ouzounis and Woese) domain (GPKOW) is a RNA-binding protein that binds to RNA via pathway modulated by PKA (protein kinase A). It is present in human spliceosome. GPKOW is also known as T54 protein or modifier of snc 2 (MOS2) homolog, and it functions as an interaction partner for Cβ2 (C subunit of PKA). The gene is found to be located on human chromosome Xp11. GPKOW protein consists of one G-patch domain and two KOW motifs. |
| Immunogen: | G patch domain and KOW motifs-containing protein recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST74012 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |