Anti-LGALS1 antibody produced in rabbit
SIGMA/HPA000646 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-β-Galactoside-binding lectin L-14-I antibody produced in rabbit; Anti-14 kDa lectin antibody produced in rabbit; Anti-Galaptin antibody produced in rabbit; Anti-Galectin-1 antibody produced in rabbit; Anti-HBL antibody produced in rabbit; Anti-HPL antibody produced in rabbit; Anti-Lactose-binding lectin 1 antibody produced in rabbit; Anti-Lectin galactoside-binding soluble 1 antibody produced in rabbit; Anti-Putative MAPK-activating protein MP12 antibody produced in rabbit; Anti-S-Lac lectin 1 antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Western blot analysis in human cell lines PC-3 and Caco-2 using Anti-LGALS1 antibody. Corresponding LGALS1 RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-LGALS1 antibody HPA000646 (A) shows similar pattern to independent antibody HPA000687 (B).
Immunohistochemistry Immunohistochemical staining of human endometrium shows strong cytoplasmic positivity in cells in endometrial stroma.
Immunohistochemistry Immunohistochemical staining of human placenta shows moderate cytoplasmic positivity in decidual cells.
Immunohistochemistry Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in dermal cells.
Immunohistochemistry Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in a subset of hepatocytes.
Western Blotting Western blot analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | EVAPDAKSFVLNLGKDSNNLCLHFN |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:500-1:1000 | |
| UniProt accession no. | P09382  |
| Application: | Anti-LGALS1 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | Galectin-1 (also known as GBP, GAL1) is a protein encoded by the LGALS1 gene in humans. It is a member of a family of multifunctional lectins, plays key roles in diverse biological processes that includes cell signaling, immunomodulation, neuroprotection and angiogenesis. It modulates tumor progression by mediating cell-cell and cell-extracellular matrix interactions, as well as angiogenesis and tumor immune-escape. The gene is associated with nasopharyngeal carcinoma (NPC), also contributes to both tumorigenesis and cisplatin resistance in epithelial ovarian cancer (EOC). Gal-1 is considered as a potential therapeutic target for EOC. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Galectin-1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST73441 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |