Anti-SNAP25 antibody produced in rabbit
SIGMA/HPA001830 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-SNAP-25 antibody produced in rabbit; Anti-SUP antibody produced in rabbit; Anti-Super protein antibody produced in rabbit; Anti-
MDL Number: MFCD00675279
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human cerebral cortex and testis tissues using HPA001830 antibody. Corresponding SNAP25 RNA-seq data are presented for the same tissues.
Enhanced Validation-Orthogonal RNAseq Confirmation Western blot analysis in human cell lines U2OS and MCF-7 using Anti-SNAP25 antibody. Corresponding SNAP25 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human lymph node shows no positivity in non-germinal center cells as expected.
Immunohistochemistry Immunohistochemical staining of human testis shows no positivity in cells in seminiferous ducts as expected.
Western Blotting Western blot analysis in mouse cerebral cortex tissue.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | SSDAYKKAWGNNQDGVVASQPARVV |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:1000-1:2500 | |
| UniProt accession no. | P60880  |
| Application: | Anti-SNAP25 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Synaptosomal associated protein of 25kDa (SNAP25) is a nerve terminal protein with two isoforms, SNAP 25a and SNAP 25b. It is widely distributed in the frontal cortex and cerebellum of adult Down syndrome brain and plays an important role in Ca2+ dependent secretory vesicle-plasma membrane fusion in neuroendocrine cells. It is a component of a neuronal SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) protein, essential for neurotransmitter release from presynaptic terminals. It binds directly to the vesicle-associated membrane protein such as syntaxin and synaptobrevin to mediate synaptobrevin binding for exocytosis. |
| Immunogen: | Synaptosomal-associated protein 25 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST78868 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |