Anti-TRAT1 antibody produced in rabbit
SIGMA/HPA002356 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-T-cell receptor-associated transmembrane adapter 1 antibody produced in rabbit; Anti-T-cell receptor-interacting molecule antibody produced in rabbit; Anti-TRIM antibody produced in rabbit; Anti-pp29/30 antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human lymph node and cerebral cortex tissues using Anti-TRAT1 antibody. Corresponding TRAT1 RNA-seq data are presented for the same tissues.
By Recombinant Expression Western Blotting. Western blot analysis in control (vector only transfected HEK293T lysate) and TRAT1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY413992).
Immunohistochemistry Immunohistochemical staining of human lymph node shows high expression.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows low expression as expected.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | RQDKMYSYSSDHTRVDEYYIEDTPI |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 0.04-0.4 μg/mL | |
| UniProt accession no. | Q6PIZ9  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | T cell receptor associated transmembrane adaptor 1 (TRAT1) plays a role in the assembly and the cell surface expression by interacting with the TCR (T cell receptor) via the TCR-ξ chain in the TCR/CD3/ξ complex. RING finger region can bind Zn ions in a way similar to the classical zinc finger motif. Its coiled-coil domain is responsible for the homomeric and heteromeric interactions among the family members as well as with other proteins. It also interacts with ubiquitin conjugating (E2) enzymes involved in ubiquitin-like modification processes, such as SUMOylation and ISGylation. It may also have roles in the post-translational protein modification such as ubiquitylation and other ubiquitin-like modifications. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | T cell receptor associated transmembrane adaptor 1 (TRAT1) is a transmembrane adaptor protein expressed in T cells. It belongs to the tripartite motif (TRIM) family. It is composed of a tripartite or RBCC motif with RING finger, one or two B-box domains and a coiled-coil domain. |
| Immunogen: | T-cell receptor-associated transmembrane adapter 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86519 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |