Anti-SLMAP antibody produced in rabbit
SIGMA/HPA002357 - Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Sarcolemmal membrane-associated protein; Anti-
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human liver, lymph node, smooth muscle and testis using Anti-SLMAP antibody HPA002357 (A) shows similar protein distribution across tissues to independent antibody HPA002358 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human smooth muscle and liver tissues using Anti-SLMAP antibody. Corresponding SLMAP RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-SLMAP antibody HPA002357 (A) shows similar pattern to independent antibody HPA002358 (B).
Immunohistochemistry Immunohistochemical staining of human smooth muscle shows high expression.
Immunohistochemistry Immunohistochemical staining of human liver shows low expression as expected.
Immunohistochemistry Immunohistochemical staining of human liver using Anti-SLMAP antibody HPA002357.
Immunohistochemistry Immunohistochemical staining of human lymph node using Anti-SLMAP antibody HPA002357.
Immunohistochemistry Immunohistochemical staining of human smooth muscle using Anti-SLMAP antibody HPA002357.
Immunohistochemistry Immunohistochemical staining of human testis using Anti-SLMAP antibody HPA002357.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
Western Blotting Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | TTDAQMDEQDLNEPLAKVSLLKALL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | Q14BN4  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | SLMAP (sarcolemma associated protein) is involved in various cellular functions such as apoptosis, protein translocation, and membrane fusion in the organelles. It acts as a novel regulator in cardiac function at the sarcoplasmic reticulum. In cardiac activity, it helps in the excitation-contraction (E-C) coupling. It exhibit tissue-specific expression and plays an important role in cardiac electrophysiology as well as contractile function in vivo. Mutation in SLMAP may cause Brugada syndrome. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | SLMAP (sarcolemma associated protein) is a member of tail-anchored proteins composed of several functional domains. The large regions of coiled-coil structure consist of a forkhead-associated domain, a RecN domain, two leucine zipper domains, and a tail-anchor domain. It is ubiquitously present in striated muscles including heart, cardiac, soleus, and smooth muscle. |
| Immunogen: | sarcolemma associated protein recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86520 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |