Anti-CFAP57 antibody produced in rabbit
SIGMA/HPA002736 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-FLJ32000; Anti-WDR65
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human fallopian tube and prostate tissues using HPA002736 antibody. Corresponding CFAP57 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human fallopian tube, liver, lymph node and prostate using Anti-CFAP57 antibody HPA002736 (A) shows similar protein distribution across tissues to independent antibody HPA028623 (B).
Immunohistochemistry Immunohistochemical staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry Immunohistochemical staining of human Fallopian tube shows strong positivity in cilia in glandular cells.
Immunohistochemistry Immunohistochemical staining of human lymph node shows no positivity in lymphoid cells as expected.
Immunohistochemistry Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | PESNLVYWLWEKQKVMAIVRIDTQN |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:50- 1:200 |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project  and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | WDR65 (WD repeat domain 65) is associated with various biological functions such as signal transduction, transcription regulation, pre-mRNA splicing, cytoskeletal assembly, and vesicular traffic. It is also associated with different human diseases. The β subunit of the protein is responsible for transducing cellular response or signal across the plasma membrane. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The heterotrimeric G protein WDR65 (WD repeat domain 65) is a member of the conservative WD repeat-containing family of proteins. It is present in all eukaryotes but absent in prokaryotes. It forms a circularized β propeller structure. The β subunit consists of two distinct regions: an amino-terminal α-helical region and WD repeat units which are arranged in a ring to form a propeller structure with seven blades. All blades are composed of antiparallel β sheet. |
| Immunogen: | cilia and flagella associated protein 57 |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86367 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |