Anti-ZNF74 antibody produced in rabbit
SIGMA/HPA003354 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Zinc finger protein 74 antibody produced in rabbit; Anti-hZNF7 antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human ovary shows nuclear positivity in ovarian stroma cells.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm and actin filaments.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | GEKPFDCSQCWKAFSCHSSLIVHQR |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | Q16587  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | ZNF74 (zinc finger protein 74) gene encodes an RNA-binding protein that preferentially binds to poly(U) and poly(G) RNA homopolymers via the zinc finger domain. It is found associated with the nuclear matrix that is involved in the replication of DNA and in RNA synthesis and processing. The protein may be involved in RNA metabolism. Mutations in this gene are associated with DiGeorge syndrome (DGS), a developmental disorder. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | ZNF74 (zinc finger protein 74) gene belongs to the transcription factor IIIA/Kruppel family and contains three exons. Exon 2 encodes an N-terminal truncated Kruppel-associated box (KRAB). Exon 3 encodes the zinc finger domain and the 3′-untranslated region (UTR). The protein encoded by this gene is approx. 64kDa and is localized to the nucleus. It is specifically expressed in neural crest-derived tissues and neuroepithelium of the spinal cord as well as in foregut endoderm epithelia (esophagus and respiratory tract). |
| Immunogen: | Zinc finger protein 74 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST73590 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |