Anti-BRPF1 antibody produced in rabbit
SIGMA/HPA003359 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-ARPC4; Anti-HOTTL; Anti-
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human testis and liver tissues using Anti-BRPF1 antibody. Corresponding BRPF1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human testis shows high expression.
Immunohistochemistry Immunohistochemical staining of human liver shows low expression as expected.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | SLAGDEATHHTEDAAEEERLVLLEN |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:500 |
| UniProt accession no. | P55201  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | BRPF1 (bromodomain and plant homeodomain finger containing, 1) gene encodes a component of the monocytic leukemic zinc finger (MOZ) histone acetyltransferase (HAT), a quaternary complex that acetylates histones. This component activates MOZ HAT by linking the MOZ catalytic subunit to the ING5 (inhibitor of growth 5) and hEaf6 subunits and promotes acetylation of histones. It also recruits Moz to active chromatin during mitosis. The MOZ HAT complex also functions in proper patterning for skeletal development. The PWWP domain of this protein associates with H3K36me3 (Trimethylation of Lys36 in histone H3) that mediates the elongation phase of transcription and functions as an epigenetic regulator of cell growth and differentiation. The effector domains of human BRPF1 are involved in gene transcription, chromatin binding and remodeling. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | BRPF1 (bromodomain and plant homeodomain finger containing, 1) protein contains plant homeodomain (PHD) fingers, a bromodomain that recognizes acetylated histones, and a proline-tryptophan-trypto |
| Immunogen: | Peregrin recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST84803 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |