Anti-MRC1 antibody produced in rabbit
SIGMA/HPA004114 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-C-type lectin domain family 13 member D; Anti-CD206 antigen; Anti-MMR; Anti-Macrophage mannose receptor 1 precursor
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, liver, lung and placenta using Anti-MRC1 antibody HPA004114 (A) shows similar protein distribution across tissues to independent antibody HPA045134 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human lung and cerebral cortex tissues using HPA004114 antibody. Corresponding MRC1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human lung shows strong cytoplasmic positivity in macrophages.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in Hofbauer cells.
Immunohistochemistry Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in Kupffer cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows no cytoplasmic positivity in neurons as expected.
Western Blotting Western blot analysis in human liver tissue.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | NEDHKRCVDAVSPSAVQTAACNQDA |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:1000-1:2500 | |
| UniProt accession no. | P22897  |
| Application: | Anti-MRC1 antibody has been used: • in immunofluorescence Ab staining • in confocal microscopy • in immunohistochemical staining |
| Biochem/physiol Actions: | Mannose receptor, C type 1 (MRC1) encodes for human mannose receptor (MR) and is a member of the C-type lectin receptors family. It recognizes and binds to Mycobacterium tuberculosis by the extracellular structure. It plays a role in antigen-presenting and maintaining a stable internal environment. It plays a critical role in controlling immune response and in regulating allergen induced allergic responses in asthma. This gene along with HSP70 family members through the receptor cytoplasmic tail may contribute to MR trafficking in macrophages. |
| Biochem/physiol Actions: | Mannose receptor, C type 1 (MRC1) is involved in phagocytosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Mannose receptor, C type 1 (MRC1) is a trans-membrane glycoprotein, that is expressed at high levels in the M2 macrophages. This functional gene has 30 exons and is of 101.74 kb. MRC1 consists of a ricin b-type lectin domain (RICIN), a fibronectin type-II domain (FN2), 8 C-type lectin-like domains (CTLDs), a single transmembrane domain (TM) and a short cytosolic domain. It is also expressed on endothelial cells and plasma membrane. This gene is located on human chromosome 10p12. |
| Immunogen: | Macrophage mannose receptor 1 precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86249 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |