Anti-IFITM1 antibody produced in rabbit
SIGMA/HPA004810 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-CD225 antigen; Anti-
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human epididymis and skeletal muscle tissues using HPA004810 antibody. Corresponding IFITM1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human testis shows no membranous positivity in cells in seminiferous ducts as expected.
Immunohistochemistry Immunohistochemical staining of human tonsil shows strong membranous positivity in non-germinal center cells.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows no membranous positivity in myocytes as expected.
Immunohistochemistry Immunohistochemical staining of human epididymis shows strong membranous positivity in ependymal cells.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Western Blotting Western blot analysis in human cell line CAPAN-2.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | MHKEEHEVAVLGAPPSTILPRSTVI |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:500-1:1000 | |
| UniProt accession no. | P13164  |
| Application: | Anti-IFITM1 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | IFITM1 (interferon induced transmembrane protein 1) is associated with several cellular processes, such as homotypic cell adhesion of interfereons and cellular anti-proliferative activities. It essentially plays a role in the formation of functional blood vessels. IFITM1 regulates tight junction assembly that stabilizes EC-EC (endothelial cells) interactions during endothelial lumen formation. It acts as a biomarker in endometrial stromal and smooth muscle tumors. It performs in the antiproliferative action of IFN-γ. The suppressed expression of IFITM1 inhibits antiproliferative property of IFN-γ as well as enhances the cell growth. Studies have been suggested that IFITM1 inhibits the extracellular signaling of the kinase. By binding, it increases the transcriptional activity of p53 and stabilizes the p53 protein through inhibition of p53 phosphorylation on threonine residue. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | IFITM1 (interferon induced transmembrane protein 1) is a member of interferon inducible transmembrane protein (IFITM) family. It is expressed in stable blood vessels of multiple organs. |
| Immunogen: | Interferon-induced transmembrane protein 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86661 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |