Anti-NDUFB5 antibody produced in rabbit
SIGMA/HPA005640 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-CI-SGDH antibody produced in rabbit; Anti-Complex I-SGDH antibody produced in rabbit; Anti-NADH dehydrogenase (ubiquinone) 1β-subcomplex subunit 5, mitochondrial precursor antibody produced in rabbit; Anti-NADH-ubiquinone oxidoreductase SGDH subunit antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human heart muscle and pancreas tissues using HPA005640 antibody. Corresponding NDUFB5 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human heart muscle shows strong positivity in mitochondria in cardiomyocytes.
Immunohistochemistry Immunohistochemical staining of human kidney shows strong positivity in mitochondria in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human pancreas shows weak positivity in mitochondria in exocrine glandular cells.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm and mitochondria.
Western Blotting Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted) Lane 5: Human liver tissue Lane 6: Human tonsil tissue
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | EGYVPEHWEYYKHPISRWIARNFYD |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | O43674  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | NADH dehydrogenase [ubiquinone] 1 β-subcomplex subunit 5 (NDUFB5), two mobile electron carriers and five multiprotein complexes (Complexes 1-5) are all involved in the production of ATP, generation of a proton gradient in the inner mitochondrial membrane and finally conversion of ADP to ATP. Overall, the function of complex 1 is to pass electrons from NADH to ubiquinone, simultaneously pumping hydrogen ions from the matrix of the mitochondria to inter-membrane space. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | NADH dehydrogenase [ubiquinone] 1 β-subcomplex subunit 5 (NDUFB5) is a subunit of complex 1 (NADH: ubiquinone oxidoreductase) which belongs to the electron transport chain. It belongs to the NDUFB5/SGDH protein family which is highly conserved in fungi, plants and mammals. NDUFB5 is located in the inner mitochondrial membrane. |
| Immunogen: | NADH dehydrogenase (ubiquinone) 1β-subcomplex subunit 5, mitochondrial precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86647 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |