Anti-ZNF18 antibody produced in rabbit
SIGMA/HPA006144 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-HDSG1; Anti-KOX11; Anti-ZKSCAN6; Anti-ZNF535; Anti-ZSCAN38; Anti-Zfp535
Product Type: Chemical
ChIP-Exo-Seq composite graph for Anti-ZNF18 (HPA006144, Lot 000046753) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University. *Please note not all antibody batches are validated for use in ChIP applications.
By Recombinant Expression Western Blotting. Western blot analysis in control (vector only transfected HEK293T lysate) and ZNF18 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408218).
Immunohistochemistry Immunohistochemical staining of human stomach shows strong cytoplasmic and nuclear positivity in glandular cells.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm, plasma membrane, cytosol and the Golgi apparatus.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | ISIQVLGQDILSEKMESPSCQVGEV |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 0.04-0.4 μg/mL | |
| UniProt accession no. | P17022  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Zinc finger protein 18 (ZNF18) is referred to as HDSG1 (Heart Development-specific Gene 1), KOX11, ZNF535, Zfp535, ZKSCAN6 (zinc finger with KRAB and SCAN domains) and ZSCAN38. It is mapped to human chromosome 17p12-p13 with 9 exons and 8 introns. This protein may have an essential role in the development of embryo heart. This gene consists of N-terminal leucine rich region, krueppel-associated box, and C-terminal three C2H2 type zinc finger motifs. Zinc finger protein genes are regulatory transcription factors and they interact directly with DNA and/or RNA. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| Immunogen: | Zinc finger protein 18 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70947 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |