Anti-NLRC4 antibody produced in rabbit
SIGMA/HPA006592 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-CARD12; Anti-CLAN; Anti-CLAN1; Anti-CLANA; Anti-CLANB; Anti-CLANC; Anti-CLAND; Anti-CLR2.1; Anti-NLR family, CARD domain containing 4; Anti-ipaf
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in Leydig cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | TDSLGNLKNLTKLIMDNIKMNEEDA |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500- 1:1000 |
| UniProt accession no. | Q9NPP4  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | NLRC4 (NLR family, CARD domain containing 4) is involved in the inflammatory responses. Activated NLR (NOD-like receptors) transports the caspase-1 within the inflammasome for its proteolytic activation. It converts the inactivated pro-IL-1 into active caspase by autoproteolysis. This multiprotein caspase-1 generates several innate immune sensors such as interleukin-1 family cytokines (IL1FCs), followed by cytokine productions and inflammatory cell death (pyroptosis). Mutation in the NLRC4 causes an auto-inflammatory syndrome of enterocolitis and autoinflammation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | NLRC4 (NLR family, CARD domain containing 4) is a cytoplasmic immune receptor protein associated with inflammatory responses. It is a member of the nucleotide-binding site and leucine-rich repeat (NBS-LRR) protein. It consists of ATP/GTPase-specific P-loop and Mg2+-binding site motifs. The NBs domain contains a nucleotide-binding pocket for ATP/dATP. |
| Immunogen: | NLR family, CARD domain containing 4 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70783 |
| Physical form: | Solution in phosphate buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |