Anti-SCGN antibody produced in rabbit
SIGMA/HPA006641 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Secretagogin antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human pancreas and fallopian tube tissues using HPA006641 antibody. Corresponding SCGN RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human pancreas shows strong cytoplasmic and nuclear positivity in islets of Langerhans.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cells in molecular layer.
Immunohistochemistry Immunohistochemical staining of human rectum shows strong cytoplasmic positivity in peripheral nerve / ganglion.
Immunohistochemistry Immunohistochemical staining of human Fallopian tube shows no positivity in glandular cells as expected.
Immunohistochemistry Immunofluorescence staining of mouse hypothalamus shows positivity in the paraventricular hypothalamic nucleus.
Immunohistochemistry Immunohistochemical staining of mouse olfactory bulb, glomerular layer shows nuclear positivity.
Immunohistochemistry Immunofluorescence staining of mouse brain shows positivity in fasciola cinereum.
Western Blotting Western blot analysis in human cerebellum tissue.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | RDLFLHHKKAISEAKLEEYTGTMMK |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500-1:1000 |
| western blot: 0.04-0.4 μg/mL | |
| UniProt accession no. | O76038  |
| Application: | Anti-SCGN antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | SCGN (Secretagogin) is mainly acting as a calcium ion sensor in the Ca2+-induced exocytosis and membrane fusion in neurons and in neuroendocrine cells. It can bind four Ca2+ ions, but interacts with only one SCGN interacting protein. It acts as a serum marker for identifying neuronal damage. It has been reported that SCGN may control the intracellular Ca2+ signaling pathway by reflecting cellular responses to the micro-environmental stimuli. Furthermore, neuroprotective effects of SCGN also have been documented in the Alzheimer′s disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | SCGN (Secretagogin) is a novel EF-hand, cell type specific, Ca-binding protein (CBP). It is expressed in the neuroendocrine cells. Specifically, its expression has been reported in the (entero)-endocrine cells and in neuroendocrine cells in the periphery and in the mammalian brain. It is involved in the regulation of intracellular Ca2+ dynamics. It contains a Ca2+ binding domain and six putative EF hand motifs. |
| Immunogen: | Secretagogin recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86799 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |