Anti-PITRM1 antibody produced in rabbit
SIGMA/HPA006753 - Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Metalloprotease 1 antibody produced in rabbit; Anti-Pitrilysin metalloproteinase 1 antibody produced in rabbit; Anti-Presequence protease, mitochondrial precursor antibody produced in rabbit; Anti-hMP1 antibody produced in rabbit; Anti-hPreP antibody produced in rabbit
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, colon, heart muscle and testis using Anti-PITRM1 antibody HPA006753 (A) shows similar protein distribution across tissues to independent antibody HPA006754 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human testis and salivary gland tissues using Anti-PITRM1 antibody. Corresponding PITRM1 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-PITRM1 antibody HPA006753 (A) shows similar pattern to independent antibody HPA006754 (B).
Immunohistochemistry Immunohistochemical staining of human testis shows high expression.
Immunohistochemistry Immunohistochemical staining of human salivary gland shows low expression as expected.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-PITRM1 antibody HPA006753.
Immunohistochemistry Immunohistochemical staining of human heart muscle using Anti-PITRM1 antibody HPA006753.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex using Anti-PITRM1 antibody HPA006753.
Immunofluorescence Immunofluorescent staining of human cell line U-251 MG shows localization to mitochondria.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | ELRSQQSKPQDASCLPALKVSDIEP |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | Q5JRX3  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | PITRM1 (Pitrilysin metallopeptidase 1) acts as a mitochondrial amyloid-β peptide (Aβ)-degrading enzyme localized in the mitochondrial matrix. Its activity is controlled by Zn+2-dependent metalloprotease inhibitor. The temporal lobe of the human brain has a high tendency of Aβ accumulation and reactive oxygen species (ROS) production. In Alzheimer′s disease, the low proteolytic activity of PITRM1 in the brain mitochondria has been reported. Studies have been suggested that high ROS production results in decreased PITRM1 activity followed by Aβ accumulation. As a result, it leads to mitochondrial toxicity and neuronal death. Thus, it proves the impact of PITRM1 in the Alzheimer′s disease. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | PITRM1 (Pitrilysin metallopeptidase 1) is a metalloendoprotease belonging to the pitrilysin family. It comprises of a conserved catalytic domain at the N-terminal region. It is highly expressed in the muscle, heart and lesser extent in the brain, pancreas, liver, lung, and placenta. |
| Immunogen: | Presequence protease, mitochondrial precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70795 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |