Anti-GPSM2 antibody produced in rabbit
SIGMA/HPA007327 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human cerebellum shows strong cytoplasmic and membranous positivity in Purkinje cells as well as in cells in molecular and granular layer.
Immunohistochemistry Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human rectum shows moderate cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | REVGDKSGELTARLNLSDLQMVLGL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:500-1:1000 | |
| UniProt accession no. | P81274   |
| Application: | Anti-GPSM2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunofluorescence (1 paper) |
| Biochem/physiol Actions: | GPSM2 (G-protein signaling modulator 2) is involved in the transduction of signals from cell surface receptors into integrated cellular responses. The protein functions in the maintenance of cell polarity and spindle orientation by anchoring the coiled-coil protein NuMA and dynein to the cell cortex during metaphase. It is essential for the segregation of cell lineage into muscle and dermis. It plays a crucial role in the development of normal hearing and any defects in this gene are associated with hearing loss. Defects in this gene have been observed in patients with Chudley-McCullough Syndrome showing brain malformations and hearing loss. It plays the role of a guanine nucleotide dissociation inhibitor towards both Gα(o)- and Gα(i)-subunits. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | G-protein signaling modulator 2 (GPSM2) gene encodes a member of a family of proteins that function in the activation of G proteins. It contains 10 leu-gly-asn (LGN) repeats at the N-terminal and four Gαi/o–Loco (GoLoco) motifs at the C-terminal. |
| Immunogen: | G-protein-signaling modulator 2 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST71269 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |