Anti-SEC23B antibody produced in rabbit
SIGMA/HPA008216 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Protein transport protein Sec23B antibody produced in rabbit; Anti-SEC23-related protein B antibody produced in rabbit
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human testis shows weak cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human fallopian tube shows weak to moderate cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows low positivity in myocytes as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | MVQVHELSCEGISKSYVFRGTKDLT |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | Q15437  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Protein transport protein Sec23 homolog B (SEC23B) is part of the coat protein complex II (COPII) which is required by proteins to be transported out of the endoplasmic reticulum (ER). It is a secretion-associated RAS-related protein 1 (SAR1) GTPase-activating protein and also plays a role in cargo recognition. Conversion of SAR1-GTP to SAR1-GDP causes its dissociation from the COPII coat. SAR1-bound SEC23B interacts with the C terminus of SEC31, creating a link between the outer-coat SEC13–SEC31 complex and the inner-coat SAR1–SEC23B–SEC24 complex. When it interacts with the outer coat, the GAP activity is stimulated and this accelerates the GTP hydrolysis of SAR1. SAR1 gets released and SEC23B interacts with the trappin protein transglutaminase (TRAPPI) complex subunits like dynactin which are all involved in vesicle tethering, fusion and transportation along microtubules. Finally, SEC23 is phosphorylated and this leads to the fusion of COPII vesicles with the target membrane. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene encoding protein transport protein Sec23 homolog B (SEC23B) is about 54kb long and located on the chromosome 20p11.23. It is composed of five domains: zinc finger domain, trunk domain, β-sheet domain, helical domain and gelsolin domain. |
| Immunogen: | Protein transport protein Sec23B recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86883 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |