Anti-NFKB2 antibody produced in rabbit
SIGMA/HPA008422 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-DNA-binding factor KBF2; Anti-H2TF1; Anti-Lymphocyte translocation chromosome 10; Anti-Lyt10; Anti-Nuclear factor NF-kappa-B p100 subunit; Anti-Oncogene Lyt-10
MDL Number: MFCD01322455
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, kidney, lymph node and testis using Anti-NFKB2 antibody HPA008422 (A) shows similar protein distribution across tissues to independent antibody HPA023900 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human appendix and skeletal muscle tissues using Anti-NFKB2 antibody. Corresponding NFKB2 RNA-seq data are presented for the same tissues.
Enhanced Validation-RNAi Knockdown Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe nos. 1 and 2, using Anti-NFKB2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunohistochemistry Immunohistochemical staining of human appendix shows high expression.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex using Anti-NFKB2 antibody HPA008422.
Immunohistochemistry Immunohistochemical staining of human kidney using Anti-NFKB2 antibody HPA008422.
Immunohistochemistry Immunohistochemical staining of human lymph node using Anti-NFKB2 antibody HPA008422.
Immunohistochemistry Immunohistochemical staining of human testis using Anti-NFKB2 antibody HPA008422.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| application(s) | research pathology |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq RNAi knockdown independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | LLNAAQNTMEPPLTPPSPAGPGLSL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | Q00653  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | Nuclear factor NF-κ-B p100 subunit (NFKB2) is at the endpoint of many of signal transduction pathways which are initiated by stimuli such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. It forms a homo- or heterodimeric complex with proteins. These complexes bind at κ-B sites in the DNA of their target genes. NFKB2 complexes are present in the cytoplasm in an inactive state bound to the NF-κ-B inhibitors. For the activation of NFKB2, I-κ-B (inhibitor) is phosphorylated by I-κ-B kinases (IKKs) and degraded. Thus the active NF-κ-B complex is free from the inhibitor and can translocate to the nucleus. NFKB2 plays an important role in the cytoplasmic retention of attached NFKB2 proteins by p100 and the generation of p52 by cotranslational processing. It also regulates the circadian clock by repressing the transcriptional activator activity of the complex formed by aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) with other proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Nuclear factor NF-κ-B p100 subunit (NFKB2) is a transcription factor which belongs to the NF-kappa B/Rel family of proteins. |
| Immunogen: | Nuclear factor NF-kappa-B p100 subunit recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86729 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |