Anti-RRBP1 antibody produced in rabbit
SIGMA/HPA009026 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-180 kDa ribosome receptor homolog antibody produced in rabbit; Anti-ES/130-related protein antibody produced in rabbit; Anti-Ribosome receptor protein antibody produced in rabbit; Anti-
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human colon, kidney, lymph node and placenta using Anti-RRBP1 antibody HPA009026 (A) shows similar protein distribution across tissues to independent antibody HPA011924 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human placenta and skeletal muscle tissues using HPA009026 antibody. Corresponding RRBP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows weak cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-RRBP1 antibody HPA009026.
Immunohistochemistry Immunohistochemical staining of human kidney using Anti-RRBP1 antibody HPA009026.
Immunohistochemistry Immunohistochemical staining of human lymph node using Anti-RRBP1 antibody HPA009026.
Immunohistochemistry Immunohistochemical staining of human placenta using Anti-RRBP1 antibody HPA009026.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
Western Blotting Western blot analysis in human cell line CACO-2.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | ELHSKLQSSEAEVRSKCEELSGLHG |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:2500-1:5000 | |
| UniProt accession no. | Q9P2E9  |
| Application: | Anti-RRBP1 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | RRBP1 (ribosome binding protein 1) is a key ribosome binding protein and plays essential role in the translocation of naïve proteins across the membrane of endoplasmic reticulum (ER). It is essential for the terminal differentiation of secretory tissues, and for the production of pro-collagen in secretory tissues. It also functions as the interacting partner of the motor protein KIF5B (kinesin family member 5B), which is up-regulated in several cancer cell lines. RRBP1 protein is highly expressed in breast cancer (BC) cells and might have potential as a BC marker. This protein is essential for the ER-localization of bulk poly(A) and of certain mRNAs such as, placental alkaline phosphatase and calreticulin. Up-regulation of this protein promotes the progression of colorectal cancer, and functions as a predictor of poor outcome in post-operative cases. In secretory cells, RRBP1-rich ER is responsible for the expansion of trans-Golgi network following the secretion of activated collagen. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | RRBP1 (ribosome binding protein 1) is a membrane protein that localizes to endoplasmic reticulum (ER). It is also found in cytoplasm and nucleus. This protein is composed of 1410 amino acids, a transmembrane domain in its hydrophobic N-terminal, a highly conserved tandem repeat which functions as the ribosome-binding domain, and the C-terminal which contains an acidic coiled-coil domain. |
| Immunogen: | Ribosome-binding protein 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST71890 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |