Anti-TIMM17A antibody produced in rabbit
SIGMA/HPA010083 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-TIM17; Anti-TIM17A; Anti-translocase of inner mitochondrial membrane 17 homolog A (yeast)
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows moderate granular cytoplasmic positivity in neurons.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunofluorescence Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm and mitochondria.
Western Blotting Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | TRFASAQFPNGPQFAEDPSQLPSTQ |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | Q99595  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | TIMM17A (translocase of inner mitochondrial membrane 17 homolog A) is a part of the inner-membrane complexes, which participate in the membrane insertion of mitochondrial proteins. This protein connects TIM23 with PAM complex, and is responsible for the dynamics of TIM23 complex. Inactivation of this protein leads to the collapse of TIM23 channel into a single pore. Thus, it is essential for maintaining pore structure and voltage gating of TIM23 channels. It prevents mitochondrial (mt)DNA instability and hence, prevents mtDNA loss. In human breast cancers, its expression is linked with unfavorable parameters including, tumor grade, NPI (Nottingham prognostic index), TNM stage and nodal positivity. Thus, in breast cancer, its expression is linked with poor prognosis. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | TIMM17A (translocase of inner mitochondrial membrane 17 homolog A) is a member of the TIM23 presequence translocase of the inner mitochondrial membrane complexes. It is highly conserved across eukaryotes. It is one of the two TIM17 genes, and is expressed in adult and fetal tissues, where it forms complexes with TIM23 protein. |
| Immunogen: | translocase of inner mitochondrial membrane 17 homolog A (yeast) recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST71954 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |