Anti-TMIGD2 antibody produced in rabbit
SIGMA/HPA011081 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Transmembrane and immunoglobulin domain-containing protein 2 precursor antibody produced in rabbit
Product Type: Chemical
By Recombinant Expression Western Blotting. Western blot analysis in control (vector only transfected HEK293T lysate) and TMIGD2 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY408250).
Immunohistochemistry Immunohistochemical staining of human duodenum shows distinct membranous positivity in glandular cells.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | CQVDQATAWERLRVKWTKDGAILCQ |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | Q96BF3  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | TMIGD2 (transmembrane and immunoglobulin domain-containing protein 2) is involved in modulating the morphology of cell, cell-cell interactions and cell migration. It increases cell-cell interaction and aggregation, along with increase in focal adhesions. It regulates angiogenesis by binding with various SH3 (SRC homology 3) domain containing proteins such as SPIN90 (SH3 protein interacting with Nck, 90kDa). SPIN90 also mediates cytoskeleton reorganization and therefore, controls cell adhesion. TMIGD2 is responsible for activation and co-stimulation of T-cells through AKT (v-Akt Murine Thymoma Viral Oncogene) pathway. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | TMIGD2 (transmembrane and immunoglobulin domain-containing protein 2) is a cell adhesion protein, which has one immunoglobulin (Ig) domain in its extracellular region, one transmembrane region and a cytoplasmic domain made of 110 amino acids which is rich in proline. It is highly expressed in endothelial and epithelial cells. This gene maps to human chromosome 19q13.3, spans 10.2kb and has 5 exons. The mRNA for this gene is found mainly in lymphoid organs, with highest expression in spleen, thymus, liver and peripheral blood lymphocyte. |
| Immunogen: | Transmembrane and immunoglobulin domain-containing protein 2 precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72192 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |