Anti-C1GALT1 antibody produced in rabbit
SIGMA/HPA011294 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Beta 1,3-galact; Anti-C1GalT1; Anti-Core 1 beta1,3-
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, kidney, liver and parathyroid gland using Anti-C1GALT1 antibody HPA011294 (A) shows similar protein distribution across tissues to independent antibody HPA012819 (B).
Immunohistochemistry Immunohistochemical staining of human parathyroid gland shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human kidney shows moderate to strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | VDTQPNVLHNDPHARHSDDNGQNHL |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:20-1:50 |
| UniProt accession no. | Q9NS00  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | C1GALT1 (core 1 synthase, glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1) catalyzes the transfer of galactose (Gal) to N-acetylgalactosamine (GalNAc) of a serine (Ser) or threonine (Thr) residue (Tn antigen) to form the Galβ1-3GalNAcα1-Ser/Thr structure. This structure is called the T antigen or core 1 structure, which acts as the precursor for maturation of mucin-type O-glycans. This protein is also involved in the control of kidney development, thrombopoiesis and angiogenesis. It is up-regulated in hepatocellular carcinoma (HCC) and is responsible for enhanced proliferation of HCC cells. This is achieved by the activation of hepatocyte growth factor (HGF) by C1GALT1, through modulation of MET O-glycosylation. It is responsible for the enhanced invasiveness of colon cancer via the modification of the O-glycosylation of FGFR2 (fibroblast growth factor receptor 2). Polymorphisms in C1GLAT1 gene are linked with Henoch-Schönlein purpura in Chinese population. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | C1GALT1 (core 1 synthase, glycoprotein-N-acetylgalactosamine 3-β-galactosyltransferase 1) is a mucin-type O-glycosyltransferase, which resides in Golgi bodies. It is a type II transmembrane protein with its N-terminal facing the cytosol. Its transmembrane domain is made of 26 amino acids, and its functional domain is made of 331 amino acids and faces the lumen. |
| Immunogen: | core 1 synthase, glycoprotein-N-acetylgala |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST71747 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |