Anti-SAP18 antibody produced in rabbit
SIGMA/HPA011352 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-2HOR0202; Anti-Cell growth-inhibiting protein 38; Anti-Histone deacetylase complex subunit SAP18; Anti-Sin3-associated polypeptide p18; Anti-Sin3-associated polypeptide, 18 kDa
MDL Number: MFCD07784891
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human testis shows nuclear positivity in cells in seminiferous ducts and Leydig cells.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm and nuclear bodies.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | VTQEEIKKEPEKPIDREKTCPLLLR |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | O00422  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | SAP18 (Sin3A-associated protein, 18kDa) facilitates transcriptional repression by Sin3-HDAC1 complex. It anchors itself to the promoter to aid in the formation of repressive complex. It is involved in the regulation of splicing through its ubiquitin-like fold. This fold is required for the assembly of a splicing-regulatory complex which includes RNPS1 (RNA-binding protein with serine-rich domain 1) and Acinus. SAP18 binds to HIV-1 integrase (IN) and recruits Sin3a-HDAC1 complex into HIV-1 virions. This is essential for early events following entry and replication of HIV-1 virions. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | SAP18 (Sin3A-associated protein, 18kDa) is a shuttling protein which forms a part of the exon junction complex (EJC). It is a part of apoptosis- and splicing-associated protein (ASAP) complex, which also includes RNPS1 (RNA-binding protein with serine-rich domain 1) and Acinus. It has a β-grasp fold similar to that found in ubiquitin. It also forms a part of the Sin3a/HDAC (Histone Deacetylase) complex. |
| Immunogen: | Histone deacetylase complex subunit SAP18 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST70940 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |