Anti-DSC2 antibody produced in rabbit
SIGMA/HPA012615 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-Desmocollin-2 precursor; Anti-Desmocollin-3; Anti-Desmosomal glycoprotein II and III
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human esophagus and cerebral cortex tissues using HPA012615 antibody. Corresponding DSC2 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human colon, esophagus, liver and tonsil using Anti-DSC2 antibody HPA012615 (A) shows similar protein distribution across tissues to independent antibody HPA011911 (B).
Immunohistochemistry Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows no positivity in neurons as expected.
Immunohistochemistry Immunohistochemical staining of human liver shows weak membranous positivity in hepatocytes.
Immunohistochemistry Immunohistochemical staining of human colon shows moderate membranous positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human tonsil shows strong membranous positivity in squamous epithelial cells.
Prestige Antibodies® Highly characterized and Enhanced Validated antibodies
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | KTVIICKPTMSSAEIVAVDPDEPIH |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:500 |
| UniProt accession no. | Q02487  |
| Application: | Anti-DSC2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . Anti-DSC2 antibody produced in rabbit can also be used for immunoprecipitation. |
| Biochem/physiol Actions: | Desmocollin 2 (DSC2) homo- and heterodimerize with each other and links the neighbouring cells by the interactions with the help of their extracellular cadherin domains. The domains also help these proteins to bind to desmogleins in a Ca2+-dependent manner. Its intracellular portions interact with plakoglobin (PG) and plakophilin-2 (PKP2). These two proteins in turn bind to desmoplakin (DSP) and provide a link to intermediate filaments. Mutations in the gene encoding DSC2 lead to arrhythmogenic right ventricular cardiomyopathy. DSC2 is essential for the establishment of early cardiac morphogenesis, normal myocardial structure and function and desmosome formation. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Desmocollin 2 (DSC2) is a type I membrane glycoprotein that is localized in the cardiac tissue and other desmosome-containing tissues. It is expressed as two different splice variants with different carboxy-termini. DSC2a isoform possesses a larger cytoplasmic domain than the DSC2b isoform. The gene encoding this protein is present on chromosome 18. DSC2 contains four extracellular amino terminal domains, that is, an extracellular anchor domain (EA), a short transmembrane domain (TM), an intracellular anchor domain (IA), and an intracellular cadherin-binding domain (ICS). It has a single transmembrane domain and a cytoplasmic tail at the carboxy terminus. |
| Immunogen: | Desmocollin-2 precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72496 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |