Anti-P2RY12 antibody produced in rabbit
SIGMA/HPA013796 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: P2RY12 Antibody - Anti-P2RY12 antibody produced in rabbit; P2Ry12 Antibody; Anti-ADP-glucose receptor; Anti-ADPG-R; Anti-P2T(AC); Anti-P2Y purinoceptor 12; Anti-P2Y(AC); Anti-P2Y(ADP); Anti-P2Y(cyc); Anti-P2Y12; Anti-P2Y12 platelet ADP receptor; Anti-SP1999
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human cerebral cortex and skeletal muscle tissues using HPA013796 antibody. Corresponding P2RY12 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong positivity in microglia.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows strong positivity in microglia.
Immunohistochemistry Immunohistochemical staining of human lung shows moderate to strong membranous positivity in macrophages.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows no positivity in myocytes as expected.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | LTNRQPRDKNVKKCSFLKSEFGLVW |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:20-1:50 |
| UniProt accession no. | Q9H244  |
| Application: | Anti-P2RY12 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Application: | The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. |
| Biochem/physiol Actions: | The gene P2RY12 (purinergic receptor P2Y, G-protein coupled, 12) encodes a G-protein coupled receptor for ADP and ATP coupled to G-proteins that are involved in the negative regulation of adenylyl cyclase second messenger system. It functions in normal platelet aggregation and blood coagulation. Defects in this gene may cause bleeding disorder, platelet type 8 (BDPLT8). It serves as a potential target for the treatment of thromboembolisms and other clotting disorders. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Purinergic receptor (P2Y12) is expressed in platelets. P2Y12 is mapped to human chromosome 3q25.1. It belongs to the P2Y receptors family and is predominantly expressed in astrocytes. P2Y12 is predicted to have three ATP binding regions. It has seven transmembrane regions. |
| Immunogen: | P2Y purinoceptor 12 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72772 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |