Anti-CYTH3 antibody produced in rabbit
SIGMA/HPA013979 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-ARNO3; Anti-GRP1; Anti-PSCD3; Anti-cytohesin-3
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.
Immunohistochemistry Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and in leydig cells.
Immunohistochemistry Immunohistochemical staining of human liver shows no positivity as expected.
Western Blotting Western blot analysis in human cell line U-2 OS.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | DEDGGGEGGGVPEDLSLEEREELLD |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | O43739  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Cytohesin 3, encoded by the gene CYTH3, catalyzes the exchange of guanosine-5’-diphosphate (GDP) for guanosine-5’-triphosphate (GTP). This exchange activates Arf proteins, such as Arf1 and Arf6. Cytohesin 3 participates in actin cytoskeleton rearrangement, integrin β1 recycling, and may regulate the protrusive actin rearrangements that occur in peripheral and basal regions of spreading cells. Knock-down of this protein has been found to enhance cell adhesion, spreading, and migration. It is found to be overexpressed in HCC (hepatocellular carcinoma) tissues, and may serve as a prognostic factor and therapeutic target for HCC. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene CYTH3 (cytohesin 3) encodes a protein belonging to the PSCD (pleckstrin homology, Sec7 and coiled-coil domains) family. The proteins belonging to this family contain conserved sequences and a common domain structure, the coiled coil domain at the N-terminus, a central sec7, pleckstrin homology domain at the C-terminus. Cytohesins function as guanine nucleotide exchange factors (GEFs) for ADP ribosylation factors (ARFs). The encoded protein is also referred to as GRP1 and is ubiquitously expressed. |
| Immunogen: | Cytohesin-3 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72547 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |