Anti-NECAB2 antibody produced in rabbit
SIGMA/HPA013998 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-EF-hand calcium-binding protein 2; Anti-EFCBP2 antibody produced in rabbit; Anti-N-terminal EF-hand calcium-binding protein 2; Anti-Neuronal calcium-binding protein 2; Anti-Stip-2; Anti-
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, kidney, pancreas and skin using Anti-NECAB2 antibody HPA013998 (A) shows similar protein distribution across tissues to independent antibody HPA014144 (B).
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human cerebral cortex and skin tissues using HPA013998 antibody. Corresponding NECAB2 RNA-seq data are presented for the same tissues.
Immunohistochemistry Immunofluorescence staining of mouse facial nucleus shows distinct positivity in neuronal cell bodies and processes.
Immunohistochemistry Immunofluorescence staining of mouse midbrain shows neuronal positivity in dorsal raphe.
Immunohistochemistry Immunofluorescence staining of mouse caudate putamen shows immunoreactivity in medium spiny neurons and synapses.
Immunohistochemistry Immunofluorescence staining of mouse hippocampus shows immunoreactivity in a subset of neurons,
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in a subset of neurons, as well as moderate positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human pancreas shows weak cytoplasmic positivity in islets of Langerhans.
Immunohistochemistry Immunohistochemical staining of human skin shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry Immunohistochemical staining of human kidney showsno positivity in cells in tubules as expected.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Western Blotting Western blot analysis in human cerebral cortex tissue.
Western Blotting Western blot analysis in mouse cerebral cortex tissue.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | VILDIFRRADKNDDGKLSLEEFQLF |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:1000-1:2500 | |
| UniProt accession no. | Q7Z6G3  |
| Application: | Anti-NECAB2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | The gene NECAB2 (N-terminal EF-hand calcium binding protein 2) encodes a neuronal calcium binding protein that is capable of binding to the C-terminal domain of the adenosine A2A receptor. This binding modulates cell surface expression of the receptor, the ligand-dependent internalization and the receptor-mediated activation of the MAPK (Mitogen-activated protein kinase) pathway. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene NECAB2 (N-terminal EF-hand calcium binding protein 2) encodes a member of the NECAB family of proteins containing an N-terminal EF-hand domain involved in binding of Ca2+. The EF-hand domain contains a single site that binds to calcium and is located next to a NHR domain that contains a coiled-coil domain. The N-terminus contains a bacterial domain called the DUF176 or ABM motif with unknown function. In rats, it is predominantly expressed in the brain. |
| Immunogen: | N-terminal EF-hand calcium-binding protein 2 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72715 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |