Anti-CD46 antibody produced in rabbit
SIGMA/HPA016903 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-CD46 antigen; Anti-Membrane cofactor protein precursor; Anti-TLX; Anti-Trophoblast leukocyte common antigen
MDL Number: MFCD00675791
Product Type: Chemical
Immunohistochemistry-paraffin JOURNAL CITATION: Intracardiac injection of a capsid-modified Ad5/35 results in decreased heart toxicity when compared to standard Ad5. By: Toivonen, R., Koskenvuo, J., et al. in Virol J, 2012. PubMed ID: 23190872 Image collected and cropped by CiteAb from the following publication, (http://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-296), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry-paraffin JOURNAL CITATION: Intracardiac injection of a capsid-modified Ad5/35 results in decreased heart toxicity when compared to standard Ad5. By: Toivonen, R., Koskenvuo, J., et al. in Virol J, 2012. PubMed ID: 23190872 Image collected and cropped by CiteAb from the following publication, (http://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-296), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry JOURNAL CITATION: Intracardiac injection of a capsid-modified Ad5/35 results in decreased heart toxicity when compared to standard Ad5. By: Toivonen, R., Koskenvuo, J., et al. in Virol J, 2012. PubMed ID: 23190872 Image collected and cropped by CiteAb from the following publication, (http://virologyj.biomedcentral.com/articles/10.1186/1743-422X-9-296), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human placenta and pancreas tissues using HPA016903 antibody. Corresponding CD46 RNA-seq data are presented for the same tissues.
By Recombinant Expression Western Blotting. Western blot analysis in control (vector only transfected HEK293T lysate) and CD46 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406719).
Immunohistochemistry Immunohistochemical staining of human endometrium shows weak membranous positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human prostate shows moderate membranous positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human pancreas shows no positivity in a subset of exocrine glandular cells as expected.
Immunohistochemistry Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | NGKHTFSEVEVFEYLDAVTYSCDPA |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200-1:500 |
| western blot: 0.04-0.4 μg/mL | |
| UniProt accession no. | P15529  |
| Application: | Anti-CD46 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunohistochemistry (1 paper) |
| Biochem/physiol Actions: | CD46 (CD46 molecule, complement regulatory protein) is involved in the T-cell-mediated immunity and tolerance. In presence of IL-2, CD46 acts as a costimulatory factor and induces signals for the Tr1 (T-regulatory 1)-specific cytokine phenotype in human CD4+ T cells. These stimulated CD4+ cells multiply fast to restrict the activation of bystander T cells. It also plays a major role in human spermatozoon-oocyte interaction at the oocyte plasma membrane. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | CD46 (CD46 molecule, complement regulatory protein) is a membrane cofactor protein, and behave as a complement regulatory receptor for C3b and other pathogens. |
| Immunogen: | Membrane cofactor protein precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST71847 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |