Anti-XIRP1 antibody produced in rabbit
SIGMA/HPA018458 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
Synonym: Anti-
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human heart muscle and prostate tissues using HPA018458 antibody. Corresponding XIRP1 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human heart muscle, liver, prostate and skeletal muscle using Anti-XIRP1 antibody HPA018458 (A) shows similar protein distribution across tissues to independent antibody HPA016750 (B).
Immunohistochemistry Immunohistochemical staining of human heart muscle shows moderate positivity in intercalated discs.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows weak to moderate cytoplasmic positivity in myocytes.
Immunohistochemistry Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry Immunohistochemical staining of human prostate shows no positivity in glandular cells as expected.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | RVSTEVAQLKEQTLARLLDIEEAVH |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200-1:500 |
| UniProt accession no. | Q702N8  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | Down-regulation of xin actin-binding repeat-containing protein-1 (XIRP1) in cultured chicken embryos results in abnormal development of the heart. XIRP1 is up-regulated in injured mouse muscle. The unique amino acid repeats in XIRP1 are responsible for binding and stabilization of actin-based cytoskeleton. XIRP1 interacts with phosphoglucomutase-like protein 5 (PGM5) and filamin C. All three together are present at intercalated discs of cardiac muscle and myotendinous junctions of skeletal muscle. XIRP1 also associates with the EVH1 (Ena/Vasp homology) domain proteins MENA (Protein enabled homolog) and VASP (Vasodilator-stimulated phosphoprotein). In adult heart, XIRP1 and MENA/VASP localizes with filamin C in intercalated discs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene XIRP1 (xin actin-binding repeat-containing protein-1) is mapped to human chromosome 3p22.2. It belongs to XIRP family. The protein is expressed during early developmental stages of cardiac and skeletal muscles. In adults, XIRP1 is mainly present in the intercalated discs of cardiomyocytes and the myotendinous junctions of skeletal muscle cells. The protein contains unique 16 amino acid repeats. Fluorescent tagging of the protein showed targeting to F-actin containing structures. |
| Immunogen: | Xin actin-binding repeat-containing protein 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST74653 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |