Anti-RLIM antibody produced in rabbit
SIGMA/HPA018895 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-E3 ubiquitin-protein ligase RNF12; Anti-LIM domain-interacting RING finger protein; Anti-R-LIM; Anti-RING finger LIM domain-binding protein; Anti-RING finger protein 12; Anti-RNF12; Anti-Renal carcinoma antigen NY-REN-43
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human colon shows cytoplasmic and nuclear positivity in glandular cells.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm and cytosol.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | RSESTSARPSRSERNSTEALTEVPP |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:20-1:50 |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project  and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | RLIM (RING finger LIM domain-binding protein) is an ubiquitin ligase. It binds with and degrades telomeric protein TRF1 (telomeric repeat-binding factor 1). Absence of RLIM, increases TRF1 levels and thereby results in telomere shortening. Similarly, RLIM interacts with oncoprotein stathmin and destabilizes it, thereby regulating cell proliferation in osteosarcoma cell lines. RLIM interacts with estrogen receptor α (ERα) and positively regulate the expression of endogenous ERα target genes in breast cancer cells. It is also an X chromosome inactivation activator. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene RLIM (RING finger LIM domain-binding protein) is mapped to human chromosome Xq13-q21. It belongs to RING (really interesting new gene) finger ubiquitin ligase class. The protein shuttles between the nucleus and the cytoplasm. |
| Immunogen: | E3 ubiquitin-protein ligase RNF12 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST74310 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |