Anti-THOC1 antibody produced in rabbit
SIGMA/HPA019096 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab1
Synonym: Anti-Nuclear matrix protein p84; Anti-THO complex subunit 1; Anti-Tho1
Product Type: Chemical
Enhanced Validation-Orthogonal RNAseq Confirmation Immunohistochemistry analysis in human appendix and skeletal muscle tissues using Anti-THOC1 antibody. Corresponding THOC1 RNA-seq data are presented for the same tissues.
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, colon, skeletal muscle and testis using Anti-THOC1 antibody HPA019096 (A) shows similar protein distribution across tissues to independent antibody HPA019687 (B).
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-THOC1 antibody HPA019096 (A) shows similar pattern to independent antibody HPA019687 (B).
Immunohistochemistry Immunohistochemical staining of human appendix shows high expression.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex using Anti-THOC1 antibody HPA019096.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-THOC1 antibody HPA019096.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle using Anti-THOC1 antibody HPA019096.
Immunohistochemistry Immunohistochemical staining of human testis using Anti-THOC1 antibody HPA019096.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | VFCGRIQLFLARLFPLSEKSGLNLQ |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:20-1:50 | |
| UniProt accession no. | Q96FV9  |
| Application: | Anti-THOC1 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | THOC1 (THO complex subunit 1) is a component of THO. THO is an RNP (ribonucleoprotein) complex and is also involved in formation of larger RNP complexes like TREX (transcription export). THO participates in transcriptional elongation, transcript 3′ end formation and nuclear export. THOC1 has been shown to associate with elongating RNA polymerase II, and UAP56 (spliceosome RNA helicase DDX39B). THOC1 is up-regulated in breast tumors and is correlated with metastatic state of the tumor progression. It is also linked with prostate cancer aggressiveness. On the other hand, THOC1 suppresses cell growth in lung cancer cells. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene THOC1 (THO complex subunit 1) is mapped to human chromosome 18p11.32-p11.31. The protein shuttles between the cytoplasm and nucleus. |
| Immunogen: | THO complex subunit 1 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST73947 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |