Anti-CCL2 antibody produced in rabbit
SIGMA/HPA019163 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-C-C motif chemokine 2; Anti-HC11; Anti-MCAF; Anti-MCP-1; Anti-Monocyte chemoattractant protein 1; Anti-Monocyte chemotactic and activating factor; Anti-Monocyte chemotactic protein 1; Anti-Monocyte secretory protein JE; Anti-Small-inducible cytokine A2
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human skin shows moderate positivity in plasma.
Immunohistochemistry Immunohistochemical staining of human skeletal muscle shows positivity in myocytes.
Immunofluorescence Immunofluorescent staining of human cell line BJ shows localization to the Golgi apparatus.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | orthogonal RNAseq Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | PDAINAPVTCCYNFTNRKISVQRLA |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 0.25-2 μg/mL |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | P13500  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Application: | Anti-CCL2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | Proteins belonging to chemoattractant cytokines family mainly induce the migration and accumulation of different white blood cells. CCL2 (C-C motif chemokine 2) signaling via CCR2 (C-C chemokine receptor type 2) is crucial for recruitment of macrophages to tissue lesions or inflammation sites. CCL2 is up-regulated in luminal B breast cancer cells. It controls cell growth, inhibits necrosis and autophagy. CCL2 participates in activation of spinal microglia through PI3K/AKT (phosphatidylinositol 3-kinase/protein Kinase B) signaling pathway in bone cancer pain (BCP). Isoglutaminyl cyclase mediated CCL2 maturation in brain causes neuro-inflammation in Alzheimer′s disease. Expression levels of CCL2 in synovial fluid are linked with pain and physical disability in osteoarthritis patients. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | CCL2 is a dimer and structurally comprises a triple-stranded antiparallel β-sheet, greek key motif and an irregular β- strand at N-terminus. |
| General description: | The gene CCL2 (C-C motif chemokine 2) is mapped to human chromosome 17q11.2-q12. It belongs to chemoattractant cytokines family. CCL2 is also referred to as MCP1 (monocyte chemoattractant protein 1). |
| Immunogen: | C-C motif chemokine 2 Precursor recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST86662 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |