Anti-FGD5 antibody produced in rabbit
SIGMA/HPA019191 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
Synonym: Anti-FYVE, RhoGEF and PH domain-containing protein 5; Anti-Zinc finger FYVE domain-containing protein 23
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | MLQHLNLDFHGAVMRALDDMDHEGR |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:20- 1:50 |
| UniProt accession no. | Q6ZNL6  |
| Application: | Anti-FGD5 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . The antibody has been used for immunocytochemistry in monolayer-cultured HUVECs (human umbilical vein endothelial cells). |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Immunocytochemistry (1 paper) |
| Biochem/physiol Actions: | Increase in levels of FGD5 (FYVE, RhoGEF and PH domain-containing protein 5) increases CDC42 (cell division cycle 42) activity. In vascular endothelial cells, FGD5 controls the proangiogenic action of VEGF (vascular endothelial growth factor). It modulates phosphatidylinositol 3-kinase signaling and thereby controls endothelial adhesion, survival and angiogenesis. FGD5 also participates in endothelial cell-targeted apoptosis and regulates vascular pruning. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene FGD5 (FYVE, RhoGEF and PH domain-containing protein 5) is mapped to human chromosome 3p25.1. It belongs to FGD family of guanine nucleotide exchange factors. In human umbilical vein endothelial cells, FGD5 is present at peripheral membrane ruffles and perinuclear regions. The protein contains a glutamate-rich amino-terminal region, a Dbl-homology (DH) domain, a first pleckstrin homology (PH) domain, an FYVE (Fab 1, YOTB, Vac 1 and EEA1) domain and a second carboxyl-terminal PH domain. |
| Immunogen: | FYVE, RhoGEF and PH domain-containing protein 5 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST72676 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |