Anti-SMARCC2 antibody produced in rabbit
SIGMA/HPA021213 - affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-BRG1-associated factor 170; Anti-SWI/SNF complex 170 kDa subunit; Anti-SWI/SNF complex subunit SMARCC2; Anti-SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-SMARCC2 antibody HPA021213 (A) shows similar pattern to independent antibody HPA061788 (B).
Immunohistochemistry Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
Western Blotting Western blot analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | AFGLESSGIAGTTSDEPERIEESGN |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:200-1:500 | |
| UniProt accession no. | Q8TAQ2  |
| Application: | Anti-SMARCC2 antibody produced in rabbit, a Prestige Antibody, is developed and validated by the Human Protein Atlas (HPA) project . Each antibody is tested by immunohistochemistry against hundreds of normal and disease tissues. These images can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. The antibodies are also tested using immunofluorescence and western blotting. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige . |
| Biochem/physiol Actions: | SWI/SNF (SWItch/Sucrose Non-Fermentable) chromatin-remodeling complexes are important for binding of transcription factors to nucleosomal DNA. SMARCC2 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2) is a core subunit of SWI/SNF. It is suggested that SMARCC2 is required for interaction of SWI/SNF complex with zinc finger DNA-binding domain structures, thereby bringing the complex to nucleosomal sites. SMARCC2 and BAF155 (BRG1-associated factor 155) regulate the protein levels of BAF57 and also support the stoichiometry of the SWI/SNF complex. SMARCC2 is up-regulated in HIV (human immunodeficiency virus)-1 infected cells. Absence of SMARCC2 inhibits viral activation in promyelocytic cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene SMARCC2 (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2) is mapped to human chromosome 12q13.2. SMARCC2 is commonly referred to as BAF170 (BRG1-associated factor 170). |
| Immunogen: | SWI/SNF complex subunit SMARCC2 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST73718 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |