Anti-TRIM65 antibody produced in rabbit
SIGMA/HPA021578 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
Synonym: TRIM65 Antibody - Anti-TRIM65 antibody produced in rabbit; Trim65 Antibody; Anti-Tripartite motif-containing protein 65
Product Type: Chemical
Western Blotting JOURNAL CITATION: ARRDC4 regulates enterovirus 71-induced innate immune response by promoting K63 polyubiquitination of MDA5 through TRIM65. By: Meng, J., Yao, Z., et al. in Cell Death Dis, 2017. PubMed ID: 28594402 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/cddis.2017.257), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Western Blotting JOURNAL CITATION: ARRDC4 regulates enterovirus 71-induced innate immune response by promoting K63 polyubiquitination of MDA5 through TRIM65. By: Meng, J., Yao, Z., et al. in Cell Death Dis, 2017. PubMed ID: 28594402 Image collected and cropped by CiteAb from the following publication, (http://www.nature.com/doifinder/10.1038/cddis.2017.257), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunohistochemistry Immunohistochemical staining of human skin shows moderate cytoplasmic positivity in squamous epithelial cells.
Immunohistochemistry Immunohistochemical staining of human placenta shows weak cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry Immunohistochemical staining of human colon shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Western Blotting Western blot analysis in human cell line U-138MG.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| immunogen sequence | LELFCRTEGRCVCSVCTVRECRLHE |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| UniProt accession no. | Q6PJ69  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Biochem/physiol Actions: | Ubiquitination of TNRC6 proteins is through E3 ligase activity of TRIM65. The coiled coil domain plays a role in protein-protein interactions and oligomerization. It is associated with repression of miRNA activity. TRIM65 colludes and adjoins with trinucleotide repeat TNRC6 (trinucleotide repeat-containing gene 6) proteins. Genomic studies on cerebral white matter lesions have found a new locus on chromosome 17q25 accommodating TRIM65. The studies also determine a probable mechanism leading to these lesions. Uncontrolled TRIM65 activities extirpate TNRC6 stability and alter RISC (RNA-induced silencing complex) stability, miRNA activity and cellular homeostasis. Gene silencing of TRIM65 with that of siRNA leads to attenuation of let-7a reporter activity. An abnormal expression of TRIM65 has a similar effect. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | Tripartite motif 65 (TRIM65) belongs to TRIM family proteins. TRIM family contains B-box 1 and a B-box 2 type domain. The coiled domain possesses hyper-helical regions. TRIM65 exhibits a SPRY (spla and ryanodine) domain at its C-terminus. Two anti-parallel β-sheets in the SPRY domain form a curved β-sandwich. Trim65 homologous genes have been found in mammals. |
| Immunogen: | Tripartite motif-containing protein 65 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST75679 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |