Anti-PRPF4 antibody produced in rabbit
SIGMA/HPA022248 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution, Ab2
Synonym: Anti-HPRP4; Anti-HPRP4P; Anti-PRP4; Anti-Prp4p; Anti-SNRNP60
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, endometrium, testis and tonsil using Anti-PRPF4 antibody HPA022248 (A) shows similar protein distribution across tissues to independent antibody HPA021794 (B).
Enhanced Validation-By Independent Antibodies Western blot analysis using Anti-PRPF4 antibody HPA022248 (A) shows similar pattern to independent antibody HPA021794 (B).
Immunohistochemistry Immunohistochemical staining of human endometrium shows strong nuclear positivity in glandular cells.
Immunohistochemistry Immunohistochemical staining of human tonsil shows strong nuclear positivity in non-germinal center cells.
Immunohistochemistry Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neurons.
Immunofluorescence Immunofluorescent staining of human cell line A-431 shows localization to nuclear speckles.
Western Blotting Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | ISYCHFSPNSKMLATACWSGLCKLW |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | O43172  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | PRPF4 (Pre-mRNA processing factor 4) is a spliceosomal protein highly involved in the pre-mRNA splicing. It acts as a component of U4/U5/U6 tri-snRNP complex during the formation of active core of the spliceosome. Mutation in PRPF4 causes an autosomal dominant disorder, retinitis pigmentosa (RP) characterized with progressive loss of photoreceptors. PRPF4 is important in cell mitosis and is involved in the regulation of mitotic spindle assembly. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | PRPF4 (Pre-mRNA processing factor 4) encodes a 60kDa protein (hPrp4) consisting of 522 amino acids with seven WD40 repeats at its C-terminal end. It is a member of the Ser/Thr protein kinase family and is mapped on chromosome 9q32. It is widely expressed in human tissues and in murine neuronal cells (neural retina and retinal pigmented epithelium cells). |
| Immunogen: | U4/U6 small nuclear ribonucleoprotein Prp4 recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST75265 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |