Anti-ATP6V1H antibody produced in rabbit
SIGMA/HPA023421 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-NBP1; Anti-V-ATPase 50/57 kDa subunits; Anti-V-ATPase subunit H; Anti-V-type proton ATPase subunit H; Anti-VMA13; Anti-Vacuolar proton pump subunit H; Anti-Vacuolar proton pump subunit SFD
Product Type: Chemical
By Recombinant Expression Western blot analysis in control (vector only transfected HEK293T lysate) and ATP6V1H over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY414295).
Immunohistochemistry Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Immunohistochemistry Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry Immunohistochemical staining of human fallopian tube shows strong cytoplasmic positivity in glandular cells.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to actin filaments.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | PQVLAVAAHDVGEYVRHYPRGKRVI |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:50-1:200 | |
| UniProt accession no. | Q9UI12  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | The gene ATP6V1H (ATPase H+ transporting V1 subunit H) encodes the regulatory subunit H of the V1 domain of V-ATPase. This multisubunit complex of V-ATPase functions in the acidification of intracellular compartments via the energy generated from ATP hydrolysis. Therefore, V-ATPase participates in receptor-mediated endocytosis, intracellular trafficking processes and degradation of proteins. Down-regulation of ATP6V1H gene in human pancreatic islets has been associated with type 2 diabetes. Its expression is negatively associated with HbA1c levels. It has a positive regulatory effect on glucose-stimulated insulin secretion. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene ATP6V1H (ATPase H+ transporting V1 subunit H) is mapped to human chromosome 8q11.2. It encodes a lysosomal protein with a molar mass of 50/57kDa. This protein forms the subunit of vacuolar ATPase (V-ATPase), a protein widely found in various eukaryotic endomembrane organelles. V-ATPase is a member of the rotary ATPase family, the members of which use rotary motor mechanisms for the transportation of ions across membranes. V-ATPase has two domains, V0 and V1 that function in transportation of proton and hydrolysis of ATP, respectively. The V1 domain is composed of eight subunits, A-H. |
| Immunogen: | V-type proton ATPase subunit H recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST76182 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |