Anti-RUNDC3A antibody produced in rabbit
SIGMA/HPA023548 - Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Synonym: Anti-RPIP8 protein; Anti-RUN domain-containing protein 3A; Anti-
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemical staining of human cerebral cortex, colon, hippocampus and liver using Anti-RUNDC3A antibody HPA023548 (A) shows similar protein distribution across tissues to independent antibody HPA070733 (B).
Immunohistochemistry Immunohistochemical staining of human hippocampus using Anti-RUNDC3A antibody HPA023548.
Immunohistochemistry Immunohistochemical staining of human liver using Anti-RUNDC3A antibody HPA023548.
Immunohistochemistry Immunohistochemical staining of human cerebral cortex using Anti-RUNDC3A antibody HPA023548.
Immunohistochemistry Immunohistochemical staining of human colon using Anti-RUNDC3A antibody HPA023548.
Immunofluorescence Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Western Blotting Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4
Western Blotting Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous glycerol solution |
| immunogen sequence | LKDLEAENRRLQLQLEEAAAQNQRE |
| product line | Prestige Antibodies® Powered by Atlas Antibodies |
| Quality Level | 100 |
| shipped in | wet ice |
| species reactivity | human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL |
| immunofluorescence: 0.25-2 μg/mL | |
| immunohistochemistry: 1:1000-1:2500 | |
| UniProt accession no. | Q59EK9  |
| Application: | All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry. The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information. |
| Biochem/physiol Actions: | The gene RUNDC3A (RUN domain containing 3A) encodes a protein that interacts with Rap2, a small GTP-binding protein that belongs to the Ras superfamily, via the RUN domain at the N-terminus. Rap2 may be involved in neuronal functions along with effector molecule, RUNDC3A. |
| Features and Benefits: | Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section. Every Prestige Antibody is tested in the following ways: • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues. • Protein array of 364 human recombinant protein fragments. |
| General description: | The gene RUNDC3A (RUN domain containing 3A) encodes a protein that contains the RUN domain and is mapped to human chromosome 17q21.31. The encoded protein is 400 amino acids long and is mainly expressed in the brain. |
| Immunogen: | RUN domain-containing protein 3A recombinant protein epitope signature tag (PrEST) |
| Legal Information: | Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Corresponding Antigen APREST75541 |
| Physical form: | Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |